anti il 9 antibody  (R&D Systems)

Journal: Cell reports

Article Title: MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells

doi: 10.1016/j.celrep.2024.114565

Figure Lengend Snippet:

Article Snippet: Anti-human IL-9 PE (MH9D1) , Thermo Fisher Scientific , Cat# 12-7098-42; RRID: AB_11151145.

Techniques: Purification, Control, Virus, Plasmid Preparation, Expressing, Retroviral, Recombinant, Activation Assay, Modification, Infection, Transfection, Lysis, Extraction, Western Blot, Electron Microscopy, Cell Isolation, Staining, Mutagenesis, Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Gene Expression, cDNA Synthesis, Sequencing, Software, Real-time Polymerase Chain Reaction

Journal: Frontiers in Immunology

Article Title: Increased interleukin-9 and Th9 cells in patients with refractory Graves’ disease and interleukin-9 polymorphisms are associated with autoimmune thyroid diseases

doi: 10.3389/fimmu.2024.1341749

Figure Lengend Snippet: mRNA expression of IL-9 and IRF4 in AITD patients and correlation analysis of IL-9 and IRF4 mRNA and level of Th17 related cytokines mRNA expression. (A, B) Relative expression of IL-9 (A) and IRF4 (B) in AITD group; (C) Correlation analysis of IRF4 mRNA and IL-17 mRNA in AITD group; (D, E) Relative expression of IL-9 (D) and IRF4 (E) in GD group; (F) Correlation analysis of IL-9 mRNA and IL-17 mRNA in GD group; (G) Correlation analysis of IRF4 mRNA and IL-21mRNA in GD group; (H) Relative expression of IRF4 in HT group; (I) Correlation analysis of IRF4 mRNA and IL-17 mRNA in HT group. *P<0.05; ***P<0.001.

Article Snippet: After that, these cells were incubated under a light-free environment at 4 ° C with anti-human IL-9-APC (BD Pharmingen, USA) for 30 minutes.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Increased interleukin-9 and Th9 cells in patients with refractory Graves’ disease and interleukin-9 polymorphisms are associated with autoimmune thyroid diseases

doi: 10.3389/fimmu.2024.1341749

Figure Lengend Snippet: Plasma IL-9 protein levels in AITD patients. (A) Plasma IL-9 protein levels in AITD, GD, HT and NC groups. (B) Correlation analysis of plasma IL-9 protein levels and TGAb of HT patients. TGAb, antibodies to thyroglobulin.

Article Snippet: After that, these cells were incubated under a light-free environment at 4 ° C with anti-human IL-9-APC (BD Pharmingen, USA) for 30 minutes.

Techniques:

Journal: Frontiers in Immunology

Article Title: Increased interleukin-9 and Th9 cells in patients with refractory Graves’ disease and interleukin-9 polymorphisms are associated with autoimmune thyroid diseases

doi: 10.3389/fimmu.2024.1341749

Figure Lengend Snippet: Expression of Th9 cells in PBMCs of AITD group and Expression of cytokines in supernatants of PBMCs from AITD patients after IL-9 stimulation. (A–C) Flow cytometry was used to detect the expression of Th9 cells in the PBMCs of GD, HT and NC group. (D) the expression difference of Th9 cell percentage among AITD group, GD group, HT group and NC group; (E) correlation analysis of the percentage of Th9 cells in PBMCs and TPOAb in HT group; (F) IFN-γ expression level in the supernatant of PBMCs in GD, HT and NC group. PBMCs in each group were stimulated with IL-2 (untreated) or human recombinant IL-9 protein combined with IL-2 for 48 hours, respectively. TPOAb, antibodies to thyroperoxidase (TPOAb). * P<0.05.

Article Snippet: After that, these cells were incubated under a light-free environment at 4 ° C with anti-human IL-9-APC (BD Pharmingen, USA) for 30 minutes.

Techniques: Expressing, Flow Cytometry, Recombinant

Journal: Mediators of Inflammation

Article Title: Circulating Th22 and Th9 Levels in Patients with Acute Coronary Syndrome

doi: 10.1155/2013/635672

Figure Lengend Snippet: Circulating Th22 and Th9 frequencies in each group. (a) CD4+ T cells were gated by flow cytometry. (b) Representation of intracellular cytokine staining of Th22 and Th9 from each group. (c) The frequencies of Th22 were markedly higher in patients with acute myocardial infarction (AMI), unstable angina pectoris (UAP), and stable angina pectoris (SAP) than those in the control group (Cont). (d) The frequencies of Th9 showed no differences among these groups. * P < 0.05 versus control, ** P < 0.01 versus control, # P < 0.05 versus SAP group, and ## P < 0.01 versus SAP group.

Article Snippet: The cells were incubated with FITC anti-human CD4 (eBioscience, San Diego, CA) in room temperature for 20 min. After the surface staining, the cells were stained with Alexa anti-human IL-9 and phycoerythrin (PE) anti-human IL-22 for Th9 and Th22 detection (Both antibodies are from eBioscience, San Diego, CA).

Techniques: Flow Cytometry, Staining

Journal: Mediators of Inflammation

Article Title: Circulating Th22 and Th9 Levels in Patients with Acute Coronary Syndrome

doi: 10.1155/2013/635672

Figure Lengend Snippet: Plasma IL-22 and IL-9 concentrations analysis. (a) The plasma IL-22 levels in patients with acute myocardial infarction (AMI) and unstable angina pectoris (UAP) were significantly increased compared with those of the control group (Cont) and the stable angina pectoris (SAP) group; the plasma IL-22 levels in patients with SAP were significantly increased compared with those of the control group. (b) The plasma IL-9 levels in patients with AMI and UAP were significantly increased compared with those of the control group and the SAP group; the plasma IL-9 levels in patients with SAP were significantly increased compared with those of the control group. (c) The IL-22 concentrations showed a positive correlation with the frequencies of Th22 cells. (d) The IL-9 concentrations showed a positive correlation with the frequencies of Th9 cells. * P < 0.05 versus control, ** P < 0.01 versus control, # P < 0.05 versus SAP group, and ## P < 0.01 versus SAP group.

Article Snippet: The cells were incubated with FITC anti-human CD4 (eBioscience, San Diego, CA) in room temperature for 20 min. After the surface staining, the cells were stained with Alexa anti-human IL-9 and phycoerythrin (PE) anti-human IL-22 for Th9 and Th22 detection (Both antibodies are from eBioscience, San Diego, CA).

Techniques:

Journal: Mediators of Inflammation

Article Title: Circulating Th22 and Th9 Levels in Patients with Acute Coronary Syndrome

doi: 10.1155/2013/635672

Figure Lengend Snippet: Spearman's correlation of the frequencies of Th22 and Th9 with cardiovascular risk factors.

Article Snippet: The cells were incubated with FITC anti-human CD4 (eBioscience, San Diego, CA) in room temperature for 20 min. After the surface staining, the cells were stained with Alexa anti-human IL-9 and phycoerythrin (PE) anti-human IL-22 for Th9 and Th22 detection (Both antibodies are from eBioscience, San Diego, CA).

Techniques:

Journal: Respiratory Research

Article Title: Resolution of LPS-induced airway inflammation and goblet cell hyperplasia is independent of IL-18

doi: 10.1186/1465-9921-8-24

Figure Lengend Snippet: Inflammatory mediators detected in the BALF . A The amount of LPS recovered in the bronchoalveolar lavages was measured using the Limulus Amoebocyte Assay and expressed in international endotoxin units (EU/ml). BALF from rats instilled with saline or 1000 μg LPS were analyzed for cytokines and growth factors by multiplex ELISA and levels of IL-1α, IL-1β, IL-6, TNF-α, GRO-KC and MCP-1 over 90 d post instillation are show. B. VEGF was only increased at 8 d post- instillation of 1000 μg LPS compared to saline-instilled rats. C. IL-18 and IL-9 exhibit a cyclic pattern of expression over 90 d post instillation. Bars represent group mean values ± SEM (n = 5 rats per experimental group) * = statistically different from saline-instilled controls ( P < 0.05).

Article Snippet: The wells were blocked with PBS containing 1% normal goat serum for 45 min. Rabbit anti-human IL-9 antibody (Chemicon International, Inc., Temecula, CA) was diluted to 0.5 μg/ml in blocking solution, and the plates were incubated at 37°C for 2 h, then washed with PBS.

Techniques: Multiplex Assay, Enzyme-linked Immunosorbent Assay, Expressing